Arugumentative Essay free essay sample

Patrick Henry, one of the establishing fathers of this extraordinary nation, once said â€Å"give me freedom, or give me death!† Many incredible valiant people have battled and taken care of what we presently know and appreciate as: our essential human rights and opportunities. Our nation has just settled that each American resident is qualified for these opportunities and consequently nobody can or should restrain these rights paying little heed to the individual. In light of the Constitution of the United States and the Bill of Rights there are sure opportunities Inalienable rights that we can't be denied. It is never directly for the U.S. government to restrict citizen’s opportunities. As residents of the U.S. we reserve the option to our voice or conclusion unafraid that there will be reprisal from the administration. As in Animal Farm, the animals looked for after this privilege also; â€Å"What then should we do? Why, work day and night, body and soul, for the over toss of the human race!† (Orwell 30), exhibiting the creatures want to be dealt with similarly, similar to the people. We will compose a custom article test on Arugumentative Essay or then again any comparable subject explicitly for you Don't WasteYour Time Recruit WRITER Just 13.90/page The creatures spoke to what all humans’ want: the longing to be heard and regarded without the dread that the administration will quietness them. A model from history which plainly shows why the First Amendment is important was on account of Martin Luther King, during the 1960s. Martin Luther practiced this privilege as he talked straightforwardly and uninhibitedly against the legislature, so as to stop isolation. Martin Luther exploited, â€Å"Congress will make no law, condensing opportunity of speech.† (US Constitution first Amendment) Proof is likewise there in the Declaration of Independence, the administration ought not reserve the option to constrain citizens’ opportunities due to the primary Amendment. The animals in Animal Farm have seven rules that all the animals must follow. â€Å"Whatever goes upon two legs is an enemy.† (Orwell 43). Much the same as we have the ten orders that numerous citizens’ follow, so do the animals in Animal Farm . The second Amendment of the Constitution states â€Å"to keep and bear arms,† which implies that there is an individual option to keep and remain battle ready for self-preservation. The third Amendment of the Constitution states â€Å"No officer will, in time of harmony be quartered in any house, without the assent of the proprietor, nor in time of war, however in a way to be recommended by law,† which implies that during times of harmony, the military may not house its soldiers in private homes without the assent of the proprietors. Duringâ times of war, the military may not house its soldiers in private homes aside from as per built up lawful methodology. By putting these confinements on the private quartering of warriors, the Third Amendment causes military power to non military personnel control and shields against mishandles that can be performed by standing armed forces with troopers. Much the same as the Constitution of the United States the Declaration of Indep endence assumes a major job in opportunity. The accompanying statement from Animal Farm shows that even the animals in have sorted out standards that all citizens’ and additionally animals must follow. â€Å"We pigs are brainworkers†¦day and night we are watching†¦it is for the wellbeing of you that we drink the milk and eat those apples.†(Orwell 52). Something in history that identifies with this is the Brandenberg v. Ohio. Brandenberg was the pioneer of the Ku Klux Klan; he was indicted due to damaging the Ohio criminal syndicalism law in the Brandenberg v. Ohio. Advocation of wrongdoing harm savagery, or unlawful techniques for fear mongering as a methods for achieving modern or political change. â€Å"Participating in such gatherings that advance syndicalism was precluded as well.†(www.worldbookonline.com). To summarize the contention the Constitution of Independence and the Bill of Rights are only two of numerous confirmations, demonstrating that it is never directly for the U.S. government to restrain citizens’ opportunities. We as residents reserve the privilege to do what we need, when we need, tolerating the outcomes that may accompany free activities. Regardless of whether you consider it or not, it is never directly for the U.S. government to constrain our opportunities.

The Red light Essay Example For Students

The Red light Essay Be that as it may, when Mr Kipps got ground floor, a dim, Red light denoted the entryway, which quickly spoke to the entryway as something significant. The Red light spoke to a feeling of fiendishness, and that something risky was behind the entryway. It additionally made an impression on the crowd, which was an admonition to the entertainer for him not to experience that entryway. The primary word that sprung to the crowd was quit, identifying with the shading Red. This additionally gave the play an eery and creepy impact. Mr Kipps then came to towards the entryway handle gradually, and as he continued attempting to open the entryway, this made the crowd not have any desire to look, for they were anticipating a type of a beast or apparition to show up. Following a couple of moments of him attempting to open the entryway, a chronicle of a young lady and a little youngsters shouts were played when they kicked the bucket on the horse and trap. This account made the crowd shout with fear, and filled the play with uneasiness and stun. The chronicle additionally speaks to what will befall Mr Kipps spouse and youngster when they go on a horse and trap, for later on in the play we are educated that seeing the lady in dark twice is trailed by the demise of a kid. We will compose a custom paper on The Red light explicitly for you for just $16.38 $13.9/page Request now We utilized denoting the second toward the finish of our presentation. Sarah described on the stage square, and me and Adelade sat on two seats behind the square with our backs to the crowd. We did this, so when we bung our feet on the edge of the square, the crowd would not know where it was coming from, for they couldn't see our feet. This would make pressure in the crowd, for it would identify with the lady in blacks nearness. Emily gradually strolled towards the crowd with a genuine look all over, which would make the crowd frightened, for it resembled she was seeking them. It would likewise advise them that kids were consistently the ones to bite the dust when their folks saw the lady dressed in dark. Emilys ensemble likewise got her increasingly engaged with the job, for she wore a dark hood and dress. The shading dark speaks to fate and void, which identifies with how the lady in blacks life was a tradejy, and how her children life was damned. Pretend was likewise utilized, in light of the fact that the storyteller continued changing to the job of Keckwick. Pretend is fundamentally when a specific character assumes the job of another character by taking their considerations, sentiments and feelings. This would likewise cause the crowd to feel all the more by and by included, and will assist them with comprehension about various characters. This was compelling in the play, since it helped the crowd see how alone Mr Kipps felt at Eel Marsh house, and the amount of a solace Keckwick was to him. This identifies with the passing on the horse and trap, for Mr Kipps encounters the frequenting of the youthful womans and youngs young men demise on the horse and trap. Everytime Keckwick shows up at Eel Marsh house on his horse and trap, Mr Kipps is unnerved for he thinks it is the spooky commotion he continues hearing. This passes on to the crowd how Mr Kipps doesn't understand that his youngster will be close amazing a horse and tr ap. A scene that identifies with this is toward the end when Mr Kipps spouse and child get murdered on the horse and trap. From the outset, Mr Kipps describes about how it was a dazzling, bright day, and how there were youngsters moving about on the grass with slows down selling frosts and organic products. This totally quiets the crowd down, and makes them believe that Mr Kipps issues with the lady dressed in dark are everywhere. The lighting was a surge of brilliant, white light, which would likewise loosen up the crowd, for they would anticipate that nothing terrifying should occur. .u48c50fefaa873b1c9aef0426f7805c02 , .u48c50fefaa873b1c9aef0426f7805c02 .postImageUrl , .u48c50fefaa873b1c9aef0426f7805c02 .focused content territory { min-tallness: 80px; position: relative; } .u48c50fefaa873b1c9aef0426f7805c02 , .u48c50fefaa873b1c9aef0426f7805c02:hover , .u48c50fefaa873b1c9aef0426f7805c02:visited , .u48c50fefaa873b1c9aef0426f7805c02:active { border:0!important; } .u48c50fefaa873b1c9aef0426f7805c02 .clearfix:after { content: ; show: table; clear: both; } .u48c50fefaa873b1c9aef0426f7805c02 { show: square; change: foundation shading 250ms; webkit-progress: foundation shading 250ms; width: 100%; mistiness: 1; change: murkiness 250ms; webkit-change: darkness 250ms; foundation shading: #95A5A6; } .u48c50fefaa873b1c9aef0426f7805c02:active , .u48c50fefaa873b1c9aef0426f7805c02:hover { obscurity: 1; progress: haziness 250ms; webkit-change: haziness 250ms; foundation shading: #2C3E50; } .u48c50fefaa873b1c9aef0426f7805c02 .focused content region { width: 100%; position: relati ve; } .u48c50fefaa873b1c9aef0426f7805c02 .ctaText { fringe base: 0 strong #fff; shading: #2980B9; text dimension: 16px; textual style weight: intense; edge: 0; cushioning: 0; content enhancement: underline; } .u48c50fefaa873b1c9aef0426f7805c02 .postTitle { shading: #FFFFFF; text dimension: 16px; textual style weight: 600; edge: 0; cushioning: 0; width: 100%; } .u48c50fefaa873b1c9aef0426f7805c02 .ctaButton { foundation shading: #7F8C8D!important; shading: #2980B9; outskirt: none; fringe span: 3px; box-shadow: none; text dimension: 14px; text style weight: striking; line-stature: 26px; moz-outskirt range: 3px; content adjust: focus; content improvement: none; content shadow: none; width: 80px; min-stature: 80px; foundation: url(https://artscolumbia.org/wp-content/modules/intelly-related-posts/resources/pictures/straightforward arrow.png)no-rehash; position: total; right: 0; top: 0; } .u48c50fefaa873b1c9aef0426f7805c02:hover .ctaButton { foundation shading: #34495E!important; } .u48c50 fefaa873b1c9aef0426f7805c02 .focused content { show: table; tallness: 80px; cushioning left: 18px; top: 0; } .u48c50fefaa873b1c9aef0426f7805c02-content { show: table-cell; edge: 0; cushioning: 0; cushioning right: 108px; position: relative; vertical-adjust: center; width: 100%; } .u48c50fefaa873b1c9aef0426f7805c02:after { content: ; show: square; clear: both; } READ: Assessment of Blood Brothers EssayThere is a recorded commotion of the horse and trap leaving, at that point out of nowhere the lighting goes to a truly diminish dim, and the lady in dark strides out from behind the window ornament. At that point the crowd hears the chronicle of the shouting once more, yet this time, it speaks to Stella and her youngster. This happened extremely snappy, and would of totally stunned the crowd, for no anticipation was developed towards this occasion. The diminish, dark light would of made an extremely spooky, nightmarish kind of scene, and would show how dispaired and vexed Mr Kipps felt when he understood he had lost the ones he cherished. In my presentation, I utilized pretend when I changed from the demonstration of Stella to the job of Mr Kipps. This helped me to see how Stella and her child were totally blameless casualties, and how petrifyed they felt when they were passing on and had no one around to support them. It likewise helped me to comprehend that it probably been an extremely unpleasant encounter for the lady in dark when she was detached from her unparalleled youngster, and how she felt like no one else ought to get the opportunity to settle down with an upbeat family, since she never found the opportunity to. It likewise helped me comprehend that you don't have the foggiest idea what it feels like to lose a kid until you have experienced it yourself, and that you ought to never take anybody elses youngster as your own, for vengeance will happen later on. At the point when me and Adelade were having a discussion towards the end about the lady dressed in dark, we had a white spot light on us. This underscored the lady dressed in dark, and how she influenced Mr Kipps life inwardly. It likewise helped me get that if Mr Kipps didn't remain at Eel Marsh house, he would not have looked further into Jennet Humfryes life history, and engaged with the lady dressed in Black.

15 Celebrity Book Clubs and Bookish Celebrities to Follow in 2019

15 Celebrity Book Clubs and Bookish Celebrities to Follow in 2019 If you’re struggling to decide what to read next, or can’t join an actual book club, celebrity book clubs just might be what you’re looking for. And they’re growing in popularity. Once this was a field dominated by Oprah (the queen of celebrity book clubs), but more and more famous people are interested in telling us what to read. Some celebrities have official book clubs you can participate in through Goodreads or Facebook, and some just post what they’re reading on Instagram. Either way, celebrity book clubs are a great source of inspiration for what to read next. I’ve rounded up some of the best actual celebrity book clubs plus bookish celebrities to follow on social. Actual Celebrity Book Clubs Oprah The original celebrity book club! Oprah’s Book Club was originally a segment on her talk show where she’d pick a book and discuss it with her audience. Oprah’s seal of approval helped many of these books become bestsellers. But after a few controversies (the infamous James Frey incident, Jonathan Franzen being annoyed that Oprah was getting women to read his books), Oprah switched to reading a lot of classic novels. I guess dead authors can’t be annoying. These days, Oprah’s Book Club has moved online. Her most recent pick is Michelle Obama’s Becoming. (By the way, don’t listen to Franzenâ€"Oprah has great taste!) Reese Witherspoon Elevating female voices is the stated goal of Reese’s Book Club x Hello Sunshine. Witherspoon announces a new pick each month and you can participate in the discussion on Facebook and Instagram, or just read along at home. Her latest pick is The Proposal by Jasmine Guillory. Emma Watson Emma Watson’s feminist book club, Our Shared Shelf, has an active presence on Goodreads. Participants can discuss their reading with one another and take part in all kinds of bookish discussions. This winter, they’re reading The Things I Would Tell You: British Muslim Women Write, edited by Sabrina Mahfouz. Watsons club seems to make a real effort to be intersectional, which is great to see. Emma Roberts Emma Roberts and Karah Preiss run Belletrist, a book club that also highlights independent bookstores (which is super cool). Before launching the club, Roberts got her start in the book influencing game by posting her latest reads on Instagram. Their January 2019 pick was The Dreamers by Karen Walker Thompson, the latest in a list of (I think) smart picks that showcase compelling nonfiction and buzzy fiction by women. Sarah Jessica Parker SJP has her own imprint with Hogarth Press, where she publishes titles that reflect her interests as a reader. She has also partnered with the American Library Association’s Book Club Central to create SJP’s Picks. The most recent SJP pick is Heartland by Sarah Smarsh. Andrew Luck NFL star Andrew Luck’s book club offers picks for either “Rookies” (for kids) and “Veterans” (for older readers)â€"adorable! This month’s choices are Kelly Barnhill’s The Girl Who Drank the Moon and James S.A. Corey’s Leviathan Wakes. Florence Welch This is my personal favorite celebrity book club because I want to grow up to be Florence Welch. Yes, the woman behind Florence and the Machine has her own book club! It seems to have grown organically from Florence’s personal love of reading, so it’s less slickly curated than some of the other ones on this list but is not run by Florence herself (though the books are recommended by her). New recommendations are posted to Facebook and Instagram, where anyone can read along and chime in with their thoughts. The most recent picks are two poetry collections by Hera Lindsay Bird called Hera Lindsay Bird and Pamper Me to Hell Back. Jimmy Fallon In June 2018, Fallon launched a Tonight Show summer book club. We’ll see if it happens again this summer, but the first pick was pretty great: Tomi Adeyemi’s Children of Blood and Bone. Felicia Day Actor and writer Felicia Day, along with Veronica Belmont, Kiala Kazebee and Bonnie Burton, runs the book club Vaginal Fantasy to discuss “romance genre books with strong female lead characters.” I’m here for a romance book club! You can join in the discussion on Goodreads, where they are currently reading Bitten by Kelley Armstrong. Sarah Michelle Gellar Everyone’s favorite vampire slayer posts her reads on Facebook and Twitter, usually using the hashtag #SMGbookclub. This book club isn’t as formal as the others hereâ€"it seems to be a way for SMG to share what she’s reading with her followers. Has anyone read this? Its SO good #SMGbookclub #vacationreading #TheNest pic.twitter.com/wWpBcdJvq4 Sarah Michelle (@SarahMGellar) June 24, 2016 Bookish Celebrities Unfortunately, the world of official celebrity book clubs is pretty white so far. But there are plenty of celebrities of color who share what they’re reading on social, so you can create your own book club by doing a read-along. Here they are! Shonda Rhimes We know Rhimes has excellent taste in books because she optioned Julia Quinn’s Bridgerton series, which is currently in development (hurry, please?), among a few other literary adaptations she’s producing for Netflix. Rhimes has also expanded into online content with the Shondaland website, which has great books coverage. And she’s also been known to share what she’s reading on Instagram. View this post on Instagram #IAmBecoming A post shared by Shonda Rhimes (@shondarhimes) on Nov 14, 2018 at 6:41pm PST Mindy Kaling Kaling has written two books herself, so we know she likes books…and she often shares her picks on Instagram. View this post on Instagram When you love a girl so much you match her book. Go Busy go! @busyphilipps #ThisWillOnlyHurtALittle #CurrentlyReading A post shared by Mindy Kaling (@mindykaling) on Oct 3, 2018 at 7:07am PDT Roxane Gay Gay is one of our foremost cultural critics and essayists, in addition to being a kickass fiction writer, so you can trust her opinions. She periodically posts lists of recommended books on Medium or  her Tumblr,  and you can also follow her on Goodreads, where she writes detailed and thoughtful reviews of what she’s reading. Barack Obama Ah, remember when we could be confident that the president could read? Obama used to share his yearly book picks during his presidency and has continued the tradition now that he’s no longer in the White House. Follow him on Facebook to see the lists. (And check out Book Riot’s guide to reading like Barack!) Michelle Obama Like her husband, Michelle Obama is a reader (and now an author). She’s been known to recommend books in interviews. Book Riot has a list of a few of her past recommendations. For more bookish celebrities, check out this Book Riot list.

The Access Of Equity Finance Through Crowdfunding - 875 Words

From almost the beginning, the idea of community and local based Slow Money ideals being served through crowdfunding was acknowledged here in Southern California. Our Slow Money SoCal founding members were working against a conservative legal backdrop and a sprawling suburbia that made local investing and the community building it entails all the more difficult. In our early days, while we measured the idea of crowdfinancing still serving the Slow Money Principles, we decided it was a legitimate sounding of our regional voice. Today, the access to equity finance through crowdfunding is just another opportunity to continue to serve ways to catalyze capital while exploring the inherent tension between the virtual world and the visceral character of a place based investing. We were broached by Kiva, the grandfather to all technology based crowdfinancing in 2013. Slow Money SoCal had worked without much success, for an approach to local investment during its first year and was ready to consider another way. Still reticent about the rules and regulations that served to inhibit a local investing story, we chose to sign on as an early Kiva Trustee hoping to get our feet wet as we continued to develop our more down to earth, place based approach. We endorsed our first Kiva loan in March 2014. In those early days of Slow Money SoCal, though some of us were comforted by the Kiva partnership and its legal sanctions, some thought the partnership was a distraction. If we were busy doingShow MoreRelatedLocal Impact On The Community1136 Words   |  5 PagesLocal Impact Communities are stronger because of the opportunities created by crowdfunding. States such as Pennsylvania struggle to discover tactics to increase revenue and jobs. Pennsylvania lacks a budget, and local municipalities are struggling (Pennsylvania, n.d.). 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Pulitzer used a single collection point to raise money from an enormousRead MoreCrowdfunding of Small Entrepreneurial Ventures10415 Words   |  42 PagesAuthors: Armin Schwienbacher Benjamin Larralde CROWDFUNDING OF SMALL ENTREPRENEURIAL VENTURES Book chapter forthcoming in Handbook of Entrepreneurial Finance (Oxford University Press) Date: September 28, 2010 (final version) Armin Schwienbacher, Professor of finance, Università © Lille Nord de France, Facultà © de Finance, Banque et Comptabilità ©, Rue de Mulhouse 2 - BP 381, F - 59020 Lille Cà ©dex (France); +33 3 20 90 75 34 ; armin.schwienbacher@univ-lille2.fr. SKEMA Business School, Avenue WillyRead MoreCrowdfunding of Small Entrepreneurial Ventures10426 Words   |  42 PagesAuthors: Armin Schwienbacher Benjamin Larralde CROWDFUNDING OF SMALL ENTREPRENEURIAL VENTURES Book chapter forthcoming in Handbook of Entrepreneurial Finance (Oxford University Press) Date: September 28, 2010 (final version) Armin Schwienbacher, Professor of finance, Università © Lille Nord de France, Facultà © de Finance, Banque et Comptabilità ©, Rue de Mulhouse 2 - BP 381, F - 59020 Lille Cà ©dex (France); +33 3 20 90 75 34 ; armin.schwienbacher@univ-lille2.fr. SKEMA Business School, Avenue Willy BrandtRead MoreHow Happy Scrappy Is A Personalised Scrapbook Creation Company1638 Words   |  7 Pagescompany. It is run through an online website allowing customers to create a virtual scrapbook. The company was extremely successful in the first year and has decided to expand. 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Cr owdfunding varies enormously in terms of the size and scope of the projects, and the range of differentRead MoreCase Study : Healthy Potion1895 Words   |  8 Pagesin the lack of an international market and interest for foreign consumers. Consequently, it is not a widely recognised enterprise. Following the rapid increase of technology in business and online shopping, Healthy Potion can promote the company through online services to meet this demand. This may be achieved by developing a company website in which allows for online orders of Healthy Potion’s product, or by simply having an online presence on social media. †¢ Market development and expansion: Learning

The Beginnings Of Cinema United States - 1931 Words

2 THE BEGINNINGS OF CINEMA UNITED STATES The first public screening of a film in the United States took place in 1896 in New York. The projector was developed by inventor Thomas Alva Edison, whose company was also the producer of the short films. Fatherhood American fiction cinema is often attributed to Edwin S. Porter, who in 1903 used an innovative technique mount 8 - minute film Assault and robbery of a train by which different fragments from different shots of the same film was together to form a narrative whole. This work became film in a very popular art form, and led to nationwide screening rooms appear small, so -called nickelodeones. David Wark Griffith, a disciple of Porter, developed the principles of this using panoramic and close - up shots, as well as parallel assemblies as a means of expression to maintain dramatic tension, which became the most important silent film pioneer States United. With his works The Birth of a Nation (1915) and Intolerance (1916) began the tradition of historical cinema in his country. 3 THE BIRTH OF HOLLYWOOD Between 1915 and 1920, the film industry gradually shifted from the east coast to Hollywood, where new studies emerged. Film production became an important economic sector and imposed his rule beyond the country s borders. Westerns, detective films, adventure, science fiction and horror, who lived a time of splendor with directors like Cecil B. De Mille, John Ford, Frank Capra, William Wyler or King: At that time theShow MoreRelatedHistory of Cinema1477 Words   |  6 PagesKatrina Farmer Pd.2 History Of Cinema What is cinema? Cinema is a film, a story captured as a set of moving pictures to be shown on a screen in a movie theatre or on television. It is a passage of expression and modern science. Cinema is the most tried and true form of storytelling. 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“Drugs Take You To Hell Disguised As Heaven” 8 Simple Words

â€Å"Drugs take you to hell disguised as heaven† 8 simple words but they mean much more, drug therapist ,Donald Lynn Frost, said this. He’s talking about how the drugs have a person feeling like they’re in heaven, but they are really just putting deeper into a own personal hell. The people of the 70’s know this feeling, in their society drugs were everywhere were a normal part of life. The 70’s culture was something that dealt with rights, rebellion, and particularly women’s rights. Women’s rights were growing, advancing, and changing; instead of staying at home women were now in the workplace. In the workplace is where women’s rights came into play and they were fighting for things such as equal salary, equal job rank, and many more. While†¦show more content†¦He then revealed to everybody else this drug and encourage people to use it, declaring it could make them smarter, feel on top of the world, and better than ever before. Nobody knew the negative effects of drugs which is why they were so popular. Because nobody knew about the negative effects and only cared about having a good time, drugs came out everywhere in society. The media was a huge part, global superstars such as Elvis Presley, Johnny Cash, and Jimi Hendrix, all did drugs. People publicly knew about the drug use and simply did not care, because they did it everyone else believed it was okay to do them (Hanson 54). However after Elvis died in 1977 due to drug use, people soon began to open their eyes to the negatives. Teenagers were another influencer of drugs, they pushed these drugs on other teens. If one person did them the next would try it and so on, only because that is what they saw and it was looked upon as cool. If a student went out everyday after school and smoked marijuana he/she was cool, if the same student or someone else knew how to get heroin they were cool. The parents were the final people who pushed drugs, if the kids/teenagers see their parents doing drugs, once again it is seen as okay to do them . Studies have shown that if the child watches the parent do things theyShow MoreRelatedEssay on Fall of Asclepius95354 Words   |  382 Pagesabout fleeing from infested areas. Everywhere was infested. There was no where anyone could go without encountering the walking plague. You know that phrase War is Hell? Well... its dead wrong. War at least has some organization to it. What was faced in the last days... by last days I mean the last days of civilization not life; itself. What was faced was hell. Everyone went ape shit insane. Everyone was killing and raping each other into oblivion, because we were under attack by creatures thatRead MoreLogical Reasoning189930 Words   |  760 PagesAttributionNonCommercial-NoDerivs 3.0 Unported License. That is, you are free to share, copy, distribute, store, and transmit all or any part of the work under the following conditions: (1) Attribution You must attribute the work in the manner specified by the author, namely by citing his name, the book title, and the relevant page numbers (but not in any way that suggests that the book Logical Reasoning or its author endorse you or your use of the work). (2) Noncommercial You may not use this work for commercial purposesRead MoreMarketing Mistakes and Successes175322 Words   |  702 Pages2009 658.800973—dc22 2008040282 ISBN-13 978-0-470-16981-0 Printed in the United States of America 10 9 8 7 6 5 4 3 2 1 PREFACE Welcome to the 30th anniversary of Marketing Mistakes and Successes with this 11th edition. Who would have thought that interest in mistakes would be so enduring? Many of you are past users, a few even for decades. I hope you will find this new edition a worthy successor to earlier editions. I think this may even be my best book. TheRead MoreMonsanto: Better Living Through Genetic Engineering96204 Words   |  385 Pages14_Hanson_3ed_SB_3869_TXT.indd 441 8/29/07 1:09:33 PM 442 INTRODUCTION A SUMMARY OF THE CASE ANALYSIS PROCESS Case analysis is an essential part of a strategic management course and is also perhaps the most entertaining part of such a course. The ‘full story’ that follows this summary gives you considerable detail about how to go about a case analysis, but for now here is a brief account. Before we start, a word about attitude – make it a real exercise. You have a set of historical facts;

John Osborne’s Gym and Squash Centre †Free Sample

Question: Discuss about John Osbornes gym and squash centre. Answer: As we can see from the video, John Osbornes Gym and Squash centre has a great part of its investments in equipments, i.e., Fixed Assets. Therefore it will be profitable for the owner to monitor the Return on assets (ROA). ROA is a common profitability ratio which measures the amount of return generated by the assets relative to their cost. It gives an idea as to how efficiently the assets of the company are used to generate earnings. It is also referred to as Return on Investment (ROI). To calculate ROA the company's annualearningsis divided by its total assets. The ROA is displayed as a percentage. The higher the percentage, the better, because then the company is earning more on less investments. Along with providing services to customers, the company also maintains stock of drinks that it sells to customers. Also, it has other inventories such as squash rackets, headbands etc. Therefore, in order to make sure that it is not overstocking or under stocking, the company needs to monitor the Stock Turnover Ratio (STO). The stock turnover ratio indicates a companys efficiency in turning its inventory into sales. It shows the duration it takes for the company to sell its entire inventory. The ideal stock turnover ratio will vary from industry to industry. A higher number would mean that a longer duration is taken to sell out all of its inventory and vice-versa. Since the company deals in perishable products (drinks), a lower number will be preferred. References: Palat, R.R. (1996). Understanding Financial Ratios in Business. New Delhi: Jaico Publishing House Vandyck, C.K. (2006). Financial Ratio Analysis:A Handy Guidebook. Bloomington, Indiana: Trafford Publishing

The Great Depression Essay Example For Students

The Great Depression Essay Outline1 Introduction2 Popular topics3 Depression essay example4 Conclusion Introduction At college or at university, students are often required to complete essays and other writing assignments on particular topics or issues. The topic and the methods usually depend on the field of studies. In the psychological field, one of the most complex and controversial issues is depression. The number of people experiencing depression is only growing over time, and thus, it is important to investigate the issue and all the related aspects from different perspectives. This article will be helpful for college students studying Psychology, Medicine, Health Care, or other related disciplines. It provides a hook for writing essays about depression by offering necessary information on a good essay about depression structure and the most popular topics concerning depression. Popular topics We will write a custom essay on The Great Depression specifically for you for only $16.38 $13.9/page Order now The study of depression involves numerous controversial and relevant topics, which can become themes for your essay about depression. For example, the essay can be focused on exploring the nature of depression and related disorders or on measuring how common and serious depression is. Besides, among the popular topics, one can choose to write a paper on The Depression Signs, The Causes and Effects of Depression, Treatment Successfully Used to Overcome Depression and other topics. Choosing the right topic is the key to success in writing an essay. Besides that, you have to develop a strong and arguable thesis in your paper. Finally, you should show your awareness of the topic at both theoretical and practical levels. Your essay can be descriptive. At the same time, like the majority of essays, it can be argumentative providing strong arguments supporting the thesis. Depression essay example Depression is a complex and multi-sided phenomenon. It is not just a feeling of sadness  that every person can have. As compared to sadness, depression can be characterized by a longer duration and higher intensity, and it strongly influences people’s everyday lives. A person with depression usually loses the ability to feel pleasure and interest to everyday activities and instead, suffers from the feelings of hopelessness. Consequently, depression can often lead to the development of thoughts about suicide. Besides, the primary symptoms of depression include a considerable increase or decrease in appetite, fatigue, and sleeping disorder. As a result, a person’s body also suffers because of the mental state. Researchers differentiate between major depression and dysthymic disorder. Patients with a major disorder are those who have experienced the mentioned disease indication for some period. At the same time, these signs are rather severe and can be experienced in an intense form. People with dysthymia disorder experience the mentioned symptoms in a less severe form typically for two years or more on the majority of days. Even though the term â€Å"depression† has been used in the scientific field only for less than a hundred years, the notions of sadness, melancholy, and depression have been long known in society. Nowadays, as more and more people are experiencing depression, the study of this disorder has significantly improved. In addition, researchers state that today, antidepressants are the most frequently prescribed medications in the United States. One of the most popular ways of treatment for patients with depression is the use of St. John’s Wort herb. Other ways of treatment that can be recommended for use are exercise, Omega-3 oils, SAMe, and 5-HTP. They can be used either as stand-alone alternatives of therapy or as complementary medicines. At the same time, the success in the use of these therapies strongly depends on individual characteristics, such as the person’s reactions to different remedies and the severity of his/her symptoms. .u1720ae3b5c0439de6a7344d124c6feac , .u1720ae3b5c0439de6a7344d124c6feac .postImageUrl , .u1720ae3b5c0439de6a7344d124c6feac .centered-text-area { min-height: 80px; position: relative; } .u1720ae3b5c0439de6a7344d124c6feac , .u1720ae3b5c0439de6a7344d124c6feac:hover , .u1720ae3b5c0439de6a7344d124c6feac:visited , .u1720ae3b5c0439de6a7344d124c6feac:active { border:0!important; } .u1720ae3b5c0439de6a7344d124c6feac .clearfix:after { content: ""; display: table; clear: both; } .u1720ae3b5c0439de6a7344d124c6feac { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u1720ae3b5c0439de6a7344d124c6feac:active , .u1720ae3b5c0439de6a7344d124c6feac:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u1720ae3b5c0439de6a7344d124c6feac .centered-text-area { width: 100%; position: relative ; } .u1720ae3b5c0439de6a7344d124c6feac .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u1720ae3b5c0439de6a7344d124c6feac .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u1720ae3b5c0439de6a7344d124c6feac .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u1720ae3b5c0439de6a7344d124c6feac:hover .ctaButton { background-color: #34495E!important; } .u1720ae3b5c0439de6a7344d124c6feac .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u1720ae3b5c0439de6a7344d124c6feac .u1720ae3b5c0439de6a7344d124c6feac-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u1720ae3b5c0439de6a7344d124c6feac:after { content: ""; display: block; clear: both; } READ: The Kite Runner By John Kite EssayTypically, depressed people use a combination of different therapies as it is supposed to produce better results. It is apparent that a person should use professional help for defining the best combination of remedies that can be recommended for use regarding a particular situation. For instance, the use of complementary medicines for depressed people is combined with psychotherapy. In the majority of cases, psychotherapy is viewed as a strongly beneficial way of treating depression. The matter is that the opportunity to talk to someone about your problems can not just lead to the symptoms relief but can help to reveal the main ground of d epression and the best ways of overcoming it. What is more, in most cases psychotherapy help patients find ways for dealing with their everyday problems. Conclusion Depression as a complex and multi-sided psychological phenomenon is a popular issue discussed in student essays. These writings usually discuss the indications of the illness as well as the effect of depression on everyday life and the severity of this illness. Researchers prove that depression can be treated if a person asks for professional help and uses a combination of therapies to fight the symptoms. With regard to that, papers about depression can also be focused on the exploration of the existing ways of medical care and their effectiveness for people with depression.

The Success of Amazon.com

The Success of Amazon.com The Success of Amazon.comIf one is preparing a list of top ten companies of the decade it would not be completed without Amazon.com. The company is considered one of the top in the e-commerce industry and has managed to continue its success. The journey towards the success and its continuous growth is huge if not phenomenal and it shows how difficult it is to even be in the competition in the e-commerce industry. It was in 1994 when the Internet was still a baby that Jeff Bezos founder and chief executive of Amazon.com started the company out of a garage in Seattle. At that time the Internet was new, few had access and on top buying online was something that people feared. But of course that did not stop Bezos even though he was up against the biggest bookseller in the real world 'Barnes and Nobles'. Amazon.com became one of the primary instruments of the e-commerce revolution.Robot Co-op, makes of 43 Things with Jeff Bezos.This year too Amazon.com turned a profit despite the fact th at peers and pundits of the industry were expecting losses and even fans thought that it is the beginning of the end for the company. So what is the big mystery behind this success story, how has Amazon able to pull it off when other can barely float. Jeffrey Bezos sheds some light; one of the most important factors behind the success is the continuous refining and re-evaluation of the strategies and tactics. Bezos knows that 'Internet' is a vast and complex place to do business, he is aware of the psyche of online consumers. Moreover he realizes that in this vast, complex and dynamic place you cannot have fixed and static goals and strategies, you have to keep changing yourself, change is permanent and Amazon has been successful just...

Law Sociology Case Study Example | Topics and Well Written Essays - 1750 words

Law Sociology - Case Study Example However, a warning has to be made on the public speaking system to ensure that no people are present when the sluice gates are opened and the water rushes in a torrent. On the afternoon of June 23, 2002, this did not happen when the sluice gates were opened. As a result two people drowned and seven were bodily injured. The case came to trial in the Ontario Court of Justice in January 200. The two accused personnel, John Tammage and Robert Bednarek were accused of 'criminal negligence.' The trial was a lengthy one and took 75 days getting over in December 2006. Both the accused, John Tammage who was a part of the management team and an electrical engineer and, Robert Bednarek who worked in the electrical department as an operator, were acquitted of the negligence charges. The court ruled that the defendants did not have any motive and their actions were devoid of any criminal intent. 0.1 Analysis: The judgment ruled in favor of the big company and expectedly did not relieve the common people. This ties in with the differential social organization theory of Sutherland in 1938. The theory states that crime is backed by some organizations. In other words, it claims that crime is inherent in certain big firms. The higher authorities or government choose to ignore it or support it. Either way, crime benefits by garnering more support and voice. This theory can be further explained with reference to the securities industry. The securities industry expects a certain amount of fraud and theft to occur every year. The industry does not react to prevent such petty crimes and thus encourages crime to exist and flourish in society. This crime is organized within the industry. Sutherland adopted the concept of social disorganisation to explain the increases in crime that accompanied the transformation of preliterate and peasant societies where "influences surrounding a perso n were steady, uniform, harmonious and consistent" to modern Western civilisation which he believed was characterised by inconsistency, conflict and un-organization (1934: 64). He also believed that the mobility, economic competition and an individualistic ideology that accompanied capitalist and industrial development had been responsible for the disintegration of the large family and homogenous neighbourhoods as agents of social control. The failure of extended kin groups expanded the realm of relationships no longer controlled by the community and undermined governmental controls leading to persistent "systematic" crime and delinquency. He also believed that such disorganisation causes and reinforces the cultural traditions and cultural conflicts that support antisocial activity. The systematic quality of the behaviour was a reference to repetitive, patterned or organised offending as opposed to random events. He depicted the law-abiding culture as dominant and more extensive tha n alternative criminogenic cultural views and capable of overcoming systematic crime if organised for that purpose (1939: 8). But because society is organised around individual and small group interests, society permits crime to persist. Sutherland concluded that "if the society is organised with reference to the values expressed in the law, the crime is eliminated; if it is not organised, crime persists and develops (1939:8). Sutherland bases the theory on a few assumptions: 1. Criminal behavior is learned in interaction with other persons in a process

Tort of Negligence Essay Example | Topics and Well Written Essays - 2750 words

Tort of Negligence - Essay Example Per say the concept of negligence doe not refer to an act. Actually it is a legal concept that tends to define the basic character of an act so as to prove it to be legally wrong. As per Blyth vs. Birmingham Waterworks Co. (1856), â€Å"Negligence is the omission to do something which a reasonable man, guided upon those considerations which ordinarily regulate the conduct of human affairs, would do, or doing something which a prudent and reasonable man would not do.† However, once the concept of negligence came within the scope of the English Tort Law, the next logical and plausible challenge was to decide as to what qualifies to be termed as ‘ordinary care† and what was to be the nature and basis of the measure required to decide as to whether an act committed by an individual, organization or a group amounted to negligence. In that context, one needs to mention the much famous concept of ‘the man on the Clapham Omnibus’. ... The ‘man on the Clapham Omnibus’ represented a hypothetical person who is in general reasonable and well educated without being qualified enough to be called a specialist (Twining 64). ‘The man on the Clapham Omnibus’ represented the standard of reasoning with which to gauge a defendant’s conduct in an English Law Civil Action for Negligence. The hallmark of this concept was that it delineated and put in place a standard for ascertaining and establishing the charges of negligence on a defendant. It established a general standard of care expected of any English citizen while performing varied aspects of one’s personal or professional life. The real beauty of this standard is that it tends to be reasonable yet simple in its approach and tends to bring the legal concept of negligence within the grasp and scope of the so called common man. The very fact that this standard of care does not expect perfection on the part of ascertainers obliterates an y possibility on the part of the defendants to wriggle out of the clutches of law by resorting to technical jargon and rigmarole. It is also immensely humane and pragmatic in the sense that it do recognizes the fact that an average person lacks the foresight to foresee any risk accruing by the dint of one’s actions. Still, it is astutely responsible and practical in the sense that it enjoins on the average person the duty to be ordinarily prudent and careful, without tending to be unexceptionally or unrealistically flawless. There is no denying the fact that even the most virtuoso experts in jurisprudence do tend to overlook and appreciate the sophistication and beauty inherent in the concept of ‘the man on the Clapham Omnibus’. However, the reality is that this legal idiom not only validated the concept

National Situation on OSH in Liberia

National Situation on OSH in Liberia Name of participant: Habib K.N Sheriff Proposals for the improvement the national situation on OSH in Liberia Introduction To protect workers health and safety, a system has to be in place to ensure that the health and safety of the employees are consistently protected void of type and class of work. This system must enforce workers protection independent of the employers will, i.e. the system must ensure that the employers doesnt act at his/her will and pleasure even if it endangers the health and safety of the employees. Currently, there exist no recognized structure/policy in Liberia; however, there are specialist organizations and government bodies with direct functions in specific OSH disciplines. Unfortunately, even these bodies are dormant. As the government is meant to protect its citizenry, they must take the lead in the protection of employees. As a significant responsibility, government is to ensure decent work for all workers which include safe work. This is not unique to Liberia; it is included in the ILO Convention on Occupational Health Services No. 161 describes the occupational health service as an integrated, comprehensive, multidisciplinary team entrusted with essentially preventive functions and responsible for advising employers, workers and their representatives in undertakings on improving health at work, improving the working environment, promoting workers health, adapting work to the physical and mental capabilities of workers, and the overall development of the structural and managerial aspects of the workplace needed for health and safety (Introduction to the National OSH System, 2015). The government of Liberia being a signatory to the Convention 155 and Recommendation 164 of Occupational Safety and Health, 1981 of the ILO must therefore develop a policy consistent with best practice and strongly supported by law and used it to enforce OSH best practice in the country. This policy must have the support most preferably of all branches of the government: an act endorse by parliament and signed into law, explained by the judiciary and enforced by the executive. In such way, the highest office i.e. the office of the President supports the document thereby giving it the much needed enforcement. Strength and weaknesses of current OSH structure of Liberia: Strength Currently, there are a number of government ministries responsible to execute various OSH duties in the Liberia. These ministries are supported by law to organized specialist teams that will assist the improvement of specific sector through engagement and enforcement. They are empower to varying extend depending on the body and task they have to perform. Some even have powers of arrest- Ministry of Gender and development has the power to arrest individuals involved in child labor practices and turn the culprit over to the Ministry of Justice for prosecution, while others have powers to issue stop order like those issued by the Ministry of Health and Social Welfare, and the Ministry of Labor. Weakness Unfortunately, there is little that is been done by each of these organization. They are either dormant or lacks the manpower and technical knowhow to execute their responsibilities. Also, the support from government is lacking in some area. A major challenge in the current structure is the definition of scope and responsibilities. Several ministries tend to repeat similar roles. This is worsened by very poor communication between line ministries on cross-functional subjects. This result to unnecessary repetition like during inspections, and multiple instructions to businesses in the implementation of OSH. Proposed structure: Foundation Policy is commonly defined as a specific, deliberate course of action adopted by a government or public body to help fulfil its mandate. Any policy must be anchored in, and derive its authority from, formal legal powers and obligations and is thus an important instrument for decision-making processes. (National OSH Governance, 2015 edition), considering this definition, OSH must be enacted as the genesis for the formation of the system all that follows. This act will be aimed at strengthening its derivatives- the resulting policies and procedure. This act will be implemented through the established structure within the national framework. There must be agencies with responsibilities to perform different roles and develop regulations and Code of practices. With the support of the act, agencies can be held liable for not implementing these regulations or failure to meet best practice as prescribe in the Code of practice. Overtime, there will be collective barging which will further aid the full implementation of the policy and where necessary, technical standards to will be given by the responsible agency/authority to ensure bottle-necks are remove. Stakeholders structure Liberia has numerous ministries with OSH responsibilities, these ministries must be properly coordinated through a Competent Authority (CA) this CA must be legislated and made semi-autonomous to ensure that it acts with neither fear nor favor. The body should be so constituted and empower to countercheck the activities of line ministries responsible for the enforcement of OSH related issues in the country. For instance, in as much as health related subject falls within the ambit of the Ministry of Health and Social Welfare (MOHSW), the CA should have the overall responsibility to check the ministry and ensure that agencies are complying with regulations. Same must apply to the ministry of Labor which oversees labor related issues. The CA will oversee a tripartite council constituted by line ministries with OSH duties, private companies and workers council; also either as a part of the workers council or subsidiary bodies are the insurance and Traditional representation. This body- tripartite council will meet at regular intervals to discuss national OSH issues, whenever such meetings are held, the CA will chair and ensure proper documentation are stored with the authority. Other major bodies: Insurance bodies Although not directed regarded when naming the tripartite council, insurance plays a cardinal role in the overall establishment and maintenance of the OSH system in a country. Naturally, proper OSH management tends to greatly benefit insurance agencies by reducing compensation they pay out and increase their income. Therefore, when insurance agencies pay special attention to OSH by supporting proactive projects- prevention projects/campaigns, there will be marked improvement in the overall OSH system of the country. Traditional bodies To achieve realistic compliance to OSH policies, it is only wise to involve stakeholders having direct impacts on both the formal and informal occupational sector. In Liberia, farming is done basically through traditional methods and involves illiterate farmers using local methods. The importance of having this group represented cannot be overemphasized. Any council without a full representation of this group is incomplete because it excludes a significant number of workers hence it is not a proper representation of the overall workforce. Moreover, formulation of effective guidelines can only be done if the nature of the problem is fully understood. To understand the problem, there must be consultation which cannot be actualized without the involvement of local farmers; therefore, it is best to have the fullest representation of those carrying on the task when reviewing issues and making decisions. Rules and directives must be applicable to the people in the region for which they are design. If the tools use by a particular group of people is not known, how does a person risk assessing the task know the hazards inherent in such task? Benefits of the competent authority: Ambiguity The CA will remove ambiguity among ministries by deciding their scope and limitations in OSH. Quite frequently, line ministries do no fully know the limitations of their OSH duties as other ministries have similar functions. For instance, the Ministry of Labor (MOL) is responsible to ensure that working conditions are decent for all workers, this also is the responsible of the MOHSW who is responsible to ensure health care is optimum the workplace and that the working conditions doesnt hamper the health of the workers. Coordination A CA will ensure better coordination through joint projects and optimizing specialist skills when and where necessary. For instance, although decent work involves both MOHSW and MOL, where health is a key indicator of decent work, better coordination between these two line ministries will assist in deciding that MOHSW takes the lead due to their specialist skills in health. Unnecessary repetition and inspection CA will define the roles and limitations of each ministry. Since ministries have repeated or closely similar roles, some inspections are carried out more than once, this result to apprehension amongst businesses and industries at which these inspections are carried out, hence much lack of cooperation. Define reporting structure As a basic responsibility of the CA, it will collect reports on a timely basis and follow-up on gaps in various agencies. This define structure will regulate line ministries and agencies in complying with established guidelines. Also, reporting will aid during auditors to cross checked what is reported against what exist in the workplace. By this means, greater focus will be placed on the needed areas and removed the need for unnecessary attention. Technical services This body must be so constituted to have the necessary technical experience to facilitate training and provide assistance to various entities on OSH related subjects. Trainings should not be limited to technical services (working at height, use of PPE, lifting rigging and slinging, manual handling etc.); it should also include administration and structure in OSH. As the OSH program is relatively new in Liberia, this body must have the needed funding from government to provide 75-90% of the training free. This will encourage greater participation of organizations especially profit making ones who view OSH as additional expenditure. Conclusion: In Liberia to achieve and maintain an OSH system, a policy must be formed and controlled by a CA. This can be achieved by forming a tripartite council which meets regularly to establish and review national OSH framework and develop a plan of action. This council headed by the CA will also be supplemented by other bodies which will have full rights- these bodies must include Insurance and Traditional bodies. Said council must be supported by an act of legislation, fully constituted by all the relevant stakeholders and have the needed technical experience to properly guide the implementation of established guidelines and code of practices. This will create a fertile environment for a fast growing OSH culture where there will be more voluntary compliance. Although voluntary compliance is sought, the overall priority is to protect workers from harm physically or mentally, therefore, the CA should be given enforcement powers to act without warning when necessary. All should be cone with a basic rule Decent/safe work for all employees. Bibliography: Occupational safety and health module 6.1 -2015 edition Occupational safety and health module 6.2 -2015 edition

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DNA isolation and amplification of ansA1 and ansA3 genes Good quality genomic DNA was isolated (Sambrook, et al 2002) and bothansA1 and ansA3 genes were amplified by PCR. Clear bands of both the genes showing a size of 1kb were observed under UV transilluminator after agarose gel electrophoresis (Fig 1). Figure 1: PCR amplification of both ansA1 and ansA3 genes: Lane (A): Step up 100bp marker, Lane (B): amplification of ansA1 gene, Lane (C): amplification of ansA3 gene Overexpression and purification The overexpressed recombinant proteins (rBliAI and rBliAIII) of B. licheniformis MTCC 429 were purified to homogeneity by affinity chromatography using Ni-NTA column. Overexpression of recombinant His-tagged asparaginase into E.coli leads to improvement in yield and affinity purification process of enzyme (Enriquez et al, 2012). The fractions showing presence of protein with the help of Bradford’s reagent were pooled together. The pooled protein solution was dialyzed against the same buffer and was checked for the L-asparaginase activity. The purified protein after SDS PAGE or Native PAGE showed a single band (Fig 2) illustrating its homogeneous nature. The molecular weight of the subunits of ansA1 and ansA3was found to be approximately 37kD after SDS PAGE analysis. SDS PAGE analysis of recombinant L-asparaginase from Pyrococcus furiosus displayed single 37kDa band (Bansal et al, 2010). In another study, recombinant ansA from Rhizobium etliin SDS-PAGE showed the presence of a single polypeptide chain of 47kDa (Enriquez et al, 2012).Native PAGE analysis showed the molecular weight of the purified protein as ~74 kDa. This study confirmed that the ansA1 and ansA3 enzymes from Bacillus licheniformis MTCC 429 are a homodimer in... ...8 1.50x106 Roth et al, 2013 Table Comparative kinetic parameters for hydrolysis of L-asparagine The kinetic properties of recombinant BliA were investigated and the parameters like kcat and kcat/Km were also studied with L-asparagine as a substrate. Comparative studies of the rBliA with asparaginases from various microbial sources showed that rBliA has lower Km value for L-asparagine (Table 2) confirming thatrBliA hasbetter affinity towards its substrate than other reported (Table) microbial sources. The catalytic constant (kcat) of rBliA was only 1.5 times higher than the L-asparaginase from E.coli and catalytic constant of rErAII was 1.35 times higher than rBliA. The absolute value of kcat/Km suggests the catalytic efficiency of the enzyme (Price and Nairn, 2009). kcat/Km of the rBliAIII was 5.4x 104 which is lower than the asparaginases from E.coli and Erwinia. Essay -- DNA isolation and amplification of ansA1 and ansA3 genes Good quality genomic DNA was isolated (Sambrook, et al 2002) and bothansA1 and ansA3 genes were amplified by PCR. Clear bands of both the genes showing a size of 1kb were observed under UV transilluminator after agarose gel electrophoresis (Fig 1). Figure 1: PCR amplification of both ansA1 and ansA3 genes: Lane (A): Step up 100bp marker, Lane (B): amplification of ansA1 gene, Lane (C): amplification of ansA3 gene Overexpression and purification The overexpressed recombinant proteins (rBliAI and rBliAIII) of B. licheniformis MTCC 429 were purified to homogeneity by affinity chromatography using Ni-NTA column. Overexpression of recombinant His-tagged asparaginase into E.coli leads to improvement in yield and affinity purification process of enzyme (Enriquez et al, 2012). The fractions showing presence of protein with the help of Bradford’s reagent were pooled together. The pooled protein solution was dialyzed against the same buffer and was checked for the L-asparaginase activity. The purified protein after SDS PAGE or Native PAGE showed a single band (Fig 2) illustrating its homogeneous nature. The molecular weight of the subunits of ansA1 and ansA3was found to be approximately 37kD after SDS PAGE analysis. SDS PAGE analysis of recombinant L-asparaginase from Pyrococcus furiosus displayed single 37kDa band (Bansal et al, 2010). In another study, recombinant ansA from Rhizobium etliin SDS-PAGE showed the presence of a single polypeptide chain of 47kDa (Enriquez et al, 2012).Native PAGE analysis showed the molecular weight of the purified protein as ~74 kDa. This study confirmed that the ansA1 and ansA3 enzymes from Bacillus licheniformis MTCC 429 are a homodimer in... ...8 1.50x106 Roth et al, 2013 Table Comparative kinetic parameters for hydrolysis of L-asparagine The kinetic properties of recombinant BliA were investigated and the parameters like kcat and kcat/Km were also studied with L-asparagine as a substrate. Comparative studies of the rBliA with asparaginases from various microbial sources showed that rBliA has lower Km value for L-asparagine (Table 2) confirming thatrBliA hasbetter affinity towards its substrate than other reported (Table) microbial sources. The catalytic constant (kcat) of rBliA was only 1.5 times higher than the L-asparaginase from E.coli and catalytic constant of rErAII was 1.35 times higher than rBliA. The absolute value of kcat/Km suggests the catalytic efficiency of the enzyme (Price and Nairn, 2009). kcat/Km of the rBliAIII was 5.4x 104 which is lower than the asparaginases from E.coli and Erwinia.

Focus paper: Teenage pregnancy Essay

There are many issues that are arising in the United States. These issues seem to catch media attention but they are publicized so much that teens believe it is all in fun. They don’t understand that doing drugs does not lead to fun partying. Becoming a dealer is not a quick way to cash it’s a quick way to death. Committing suicide does not make everything easier. The only person you took the pain away from was yourself. The media captures all these fun moments that people do with only a little bit of the bad. Teenager’s girls see all these other girls on T.V raising a baby and they think â€Å"hey I can do that.† Then reality kicks in and it is nothing like how it was on T.V. There are many issues among teens in the United States, but the focus on this paper is going to be following teenage pregnancy. In today’s society when someone hears that a young girl is pregnant they automatically think she was sleeping around. People jump to the conclusions and they don’t take time to realize there are many other causes for teenage pregnancy than the girl sleeping around. The reality of it is there are actually five major causes to teenage pregnancy. Peer pressure, underage drinking, glamorizing teen pregnancy, lack of knowledge and sexual rape are the five major causes of teenage pregnancy. Peer pressure is a cause for almost anything involving teenagers; whether it be for drugs, drinking or sex. Teenagers often feel pressure to make friends and fit in. Many times these teens let their friends influence their decision to have sex even when they do not fully understand the consequences. Teenagers have sex as a way to appear cool and to fit in, but in some cases the end result is an unplanned teen pregnancy. According to the Kaiser Family Foundation states that more than 29 per cent of pregnant teens reported that they felt pressured to have sex, and 33 percent of pregnant teens stated that they felt that they were not ready for a sexual relationship, but proceeded anyway. When peer pressure is involved to have sex that usually means that drinking is involved as well. Underage drinking is another cause that leads  to teenage pregnancy. Teen drinking can cause an unexpected pregnancy, according to the website Love to Know. Many teens experiment with drugs and alcohol. Drinking lowers a teen’s ability to control her impulses, contributing to 75 percent of pregnancies that occur between the ages 14 and 21. Approximately 91 percent of pregnant teens reported that although they were drinking at the time, they did not originally plan to have sex when they conceived. Peer pressure and underage drinking go hand in hand with teenage pregnancies. One leads to another which causes an unexpected pregnancy. Out of the other three causes glamorizing teenage pregnancy and lack of knowledge also go together. The movie industry and the media contribute to teenage pregnancy by glamorizing teen pregnancy in movies. Movies that show teen pregnancy as something to be desired encourage teens to engage in reckless sexual activity, according to ABC’s â€Å"Good Morning America.† During adolescence, teens become more focused on their appearance and how people view them. Teens see on T.V that there same age are having cute little babies so they go out and have sex without being fully educated in what that really is. Teenagers who are uneducated about sex are more likely to have an unintended pregnancy. Some teens do not fully understand the biological and emotional aspects associated with having sex, according to DailyRecord.co.uk. Many times, teens do not have the knowledge needed to make informed and responsible decisions abo ut whether or not to engage in sexual activity that can alter their life. Social media is not helping teenage pregnancy it is only causing young girls to go out and try it. The last and final cause for teenage pregnancy is rape. Teens can become pregnant as a result of sexual abuse or rape. The Guttmacher Institute states that between 43 and 62 percent of teens acknowledge that they were impregnated by an adult male, and two-thirds report that their babies’ fathers are as old as 27. Approximately 5 percent of all teen births are the result of a rape. People in society need to realize that teenage pregnancy is a big issue today. Many of these young girls don’t finish high school. Teen pregnancy greatly affects education. Only 51% of teen moms have a high school diploma. The number for those who didn’t have a teen birth is closer to 89%. Parenthood is the leading cause for teen girls dropping out of school. Teen moms are extremely unlikely to go to college. Only about 2% of young teen moms ever get a higher education degree. Statistics show that In  the United States, there are nearly 750,000 teen pregnancies each year, that’s about 2000 a day! . It’s estimated that about half of Latina and African American teenagers will be pregnant a t least once before they’re 20.Raising a baby can cost up to $10,000 in the first year alone. About 80% of teen fathers don’t marry the mom. They also contribute very little to raising the baby. On average, teen dads pay less than $800 a year on child support. Teenage pregnancy is an issue just like drug use and alcohol abuse that needs to be looked into. There is no way to stop teenage pregnancy. Talking to teens about it is not going to stop them when they are at a party and alcohol is involved. Instead contraception’s should be provided in public bathrooms, schools and encouraged to be available at home. Many believe that providing protection in schools will only encourage teens to have sex. Reality is they are going to have it whether there is protection or not, so why not provide them the right choice of using protection. Maybe if protection is provided there will be less unexpected pregnancies in the United States.

Anxiety and Competitive Sports - Free Essay Example

Sample details Pages: 3 Words: 1046 Downloads: 7 Date added: 2019/04/01 Category Psychology Essay Level High school Tags: Anxiety Essay Did you like this example? When athletes prepare for a competition or performance, mental health is often neglected and not given a second thought. The athlete will condition their body, physically readying themselves to be pushed during competition. However, it might be worthwhile if athletes asked themselves how their mental health could affect their performance. Don’t waste time! Our writers will create an original "Anxiety and Competitive Sports" essay for you Create order Various studies have been conducted to observe the ways anxiety, in particular, can alter performance from practice to competitions. Anxiety may be reported by an athlete when they are unsure of whether they can handle situations contributing stress (Hardy, Jones, Gould, 1995). Martens, et al (1990) contributed three subscales, used to describe athletes anxiety participating in various sports, onto the existing knowledge of anxiety and athletic performance. These three subscales include cognitive anxiety, somatic anxiety, and self-confidence, which all play a role in an athletes performance. This multidimensional anxiety theory suggests that cognitive anxiety will negatively effect performance if arousal is high, entailing a negative linear relation with performance (Hardy, et al., 1996). On the other hand, somatic anxiety displays a curvilinear relation and self-confidence has a positive linear relation with performance (Martens, et al., 1990). A common questionnaire that was completed in various studies was the Competitive State Anxiety Inventory-2, 30 minutes to an hour before competition (Tsopani, Dallas, Skordilis, 2011). The Competitive State Anxiety Inventory-2 measures the intensity of symptoms which in turn can determine the presence of anxiety. When participants take this CSAI-2 they respond to 27 items rating each on a 4-point scale; using 1: not at all and 4: very much so (Martens, et al., 1990). A greater presence in these three subscales are indicated by higher scores. These results help to determine how an athlete feels concerning the competition at hand and how it may affect performance. Questioning how anxiety can affect an athlete during competitive play is important to improving their skills. Performance is not only determined by physical readiness, but also by an athletes mental state. It is hypothesized that athletes who contribute time towards their mental health will perform better. Additionally, those showing little symptoms of anxiety will perform better than those showing more. Cognitive anxiety increases worry or doubt, threatening an athletes persuit and well-being. When cognitive anxiety is low is when an athlete will perform the best (Kais, Raudsepp, 2004). In the studies conducted and reported by Graham Jones (1992), Austin Swain (1992), Despoina Tsopani (2011), George Dallas (2011), Emmanouli K. Skordilis (2011), Kristjan Kais (2004) and Lennart Raudsepp (2004), each participant completed CSAI-2 30-60 min before the competition to measure cogenitive anxiety. This did not interfere with preparation routines for the players. The participants were than observed during the competitions looking for mistakes or accomplishments each player achieved (Tsopani, et al., 2011). This would later determine if the results from the CSAI-2 affected their performance. The test conducted by Jones and Swain (1992) separated the 69 subjects in two groups: high or low competitive subjects. They took athletes from various sports and compared their results. Using the results given by the participants from the CSAI-2, they determined how cognitive anxiety differed between the high and low competitive subjects. In result, the more competitive subjects reported their cognitive anxiety considerably more facilitative than the less competitive subjects, positively affecting their performance (Jones, Swain, 1992). In the study conducted by Tsopani, et al., where 86 young gymnasts were observed, the results contradicted those of Jones Swain (1992). It was shown that highly competitive athletes were lower on cognitive anxiety. Additionally, Kais Raudsepp (2004) found that their 66 participants performed more effectively when they perceived their cognitive anxiety as facilitative. Thus, Jones Swain (1992) and Kais Raudsepp (2004) both found the athletes to perform better because of their perceptions on their cognitive anxiety, although cognitive anxiety had no real affect on performance. In comparison, somatic anxiety refers to the physical symptoms of anxiety in athletes, including increased heart rate, butterflies, elevated blood pressure, and more (Kais Raudsepp, 2004). Alongside cognitive anxiety, there was no statistically significant difference between cognitive and somatic anxiety. Athletes rated and perceived these two similar to one another. Self-confidence is known as the feeling of trust in ones ability to execute a task efficiently and effectively. This aspect of the multidimensional model played a significant role on how athletes performed. The way they perceived them selves in the way they played affected their performance. Jones Swain (1992) found that the highly competitive group found their self-confidence more facilitating than the low competitive group, greatly affecting performance. Tsopani, et al. (2011) stated that the only statistically significant aspect proved to be self-confidence. Female gymnasts with higher self-confidence performed higher. Reports also showed that gymnasts with lower self-confidence showed signs of shaky stances, and unbalanced performances. Furthermore, Kais Raudsepp (2004) reported results suggesting self-confidence as a construct that can greatly influence performance above affects by cognitive or somatic anxiety. There were several limitations regarding each study conducted. Jones Swain (1992) and Tsopani et al. (2011) gathered subjects that participated in the same sport at the same level while Kais Raudsepp (2004) studied athletes from varying sports. This may cause a discrepancy when comparing the athletes anxiety and self-confidence due to the different sports. These sports may not entail the same guidelines and rules athletes are to follow. Alongside this, Tsopani et al. (2011) only examined gymnasts aged 11-12, not offering results from older and more experienced athletes. Lastly, in all three reports, the intensity of anxiety was not taken during or after the competition. This eliminates the opportunity to assess anxiety and its affect in all stages of mental performance. Taking in results from all stages of the competition may offer an improvement to predicting anxieties influence. The findings of these studies support the idea that athletes perceptions of their own anxiety offer an understanding of competitive state-anxiety. Even though the symptoms of anxiety may not affect athletes negatively does not excuse its affects in a positive aspect. These affects may even be necessary for a positive affect on performance. Although, what may be perceived as positive anxiety could be merely the states of excitement, arousal, or motivation. Additionally, self-confidence directly correlates with an athletes performance during competition. The research included all showed signs that participants who perceived themselves with higher self-confidence performed better than those in a low competitive group. Furthermore, coaches and athletes should take focus on their perceptions of anxiety and self-confidence to improve their readiness for competition.